ABSTRACT
PURPOSE: Liver metastasis is the most common type of failure in the treatment of colorectal cancer. The identification of differential expressions of genes in colorectal cancer and liver metastasis is important to differentiate the genetic mechanism of carcinogenesis and liver metastasis from that of a normal mucosa. The aim of this study is to find candidate genes playing roles in liver metastasis of colorectal cancer by using cDNA microarray. METHODS: We screened a group of genes differentially expressed in a normal mucosa and in cancer and liver metastasis by using a 4.7 K cDNA microarray chip in 8 patients with far advanced colorectal cancer from Jan 2003 to May 2004 at Kyungpook National University Hospital. RESULTS: A comparison of mRNA expressions of genes in normal mucosa vs. cancer, normal mucosa vs. liver metastasis, and cancer vs. liver metastasis, 76 and 27 known and unknown genes were significantly over-expressed in cancer and liver metastasis, respectively. Also 62 and 26 genes were down- regulated in cancer and liver metastasis. Among those genes, TIMP-1, SRY-box9, Rattus norvegicus fibronectin 1, mitotic check point regulator, etc. were constantly up- regulated in cancer or metastasis, and hsgk, etc. were down-regulated in cancer or liver metastasis. CONSLUSIONS: The cDNA microarray chip technique could be a useful for robust screening of candidate genes involved in carcinogenesis or metastasis of colorectal cancer.
Subject(s)
Animals , Humans , Rats , Carcinogenesis , Colorectal Neoplasms , DNA, Complementary , Fibronectins , Gene Expression , Liver , Mass Screening , Mucous Membrane , Neoplasm Metastasis , Oligonucleotide Array Sequence Analysis , Pilot Projects , RNA, Messenger , Tissue Inhibitor of Metalloproteinase-1ABSTRACT
Irritation(traumatic) fibroma is one of the most common soft-tissue lesions in the oral cavity and represents a reactive fibrous hyperplasia. It is usually found in adults, and sites of trauma such as the buccal and labial mucosa and tongue are commonly involved. The lesion usually present as smooth, dome-shaped or pedunculated nodules with pale pink color or color of surrounding mucosa. We report four cases of irritation fibroma. The patients were all women and in their teens, fifties, and sixties. The lesions were located in labial and buccal mucosa and tongue. Interestingly, we found HPV type 1a in one of them using polymerase chain reaction. The lesions were excised and there has been no recurrence. It is a less known disease among dermatologists despite its high prevalence. We think that this is the first report of irritation fibroma in Korean dermatologic literature.